Calcium response of helper T lymphocytes to antigen-presenting cells in a single-cell assay.

@article{Agrawal1995CalciumRO,
  title={Calcium response of helper T lymphocytes to antigen-presenting cells in a single-cell assay.},
  author={Neeta Agrawal and Jennifer J. Linderman},
  journal={Biophysical journal},
  year={1995},
  volume={69 3},
  pages={1178-90}
}
We developed a dynamic, single-cell assay involving alternating differential interference contrast and fluorescence microscopy, together with digital imaging, for both viewing the physical interaction of live helper T lymphocytes (Th cells) with antigen-presenting cells (APCs) and monitoring the increases in the intracellular free calcium concentration of… CONTINUE READING