Calcium regulation of the human PMN cytosolic 15-lipoxygenase.

  title={Calcium regulation of the human PMN cytosolic 15-lipoxygenase.},
  author={Romaine Charles Nichols and Jack Y. Vanderhoek},
  journal={Biochimica et biophysica acta},
  volume={1085 1},
Addition of tracer (pg) amounts of [3H]arachidonic acid to the 120,000 x g cytosolic fraction of human polymorphonuclear leukocytes (PMNs) produced [3H]-15-HETE, the product of the 15-lipoxygenase, as the major metabolite. In the presence of nanomolar and low micromolar amounts of calcium, [3H]-15-HETE formation was increased as much as 15-fold which corresponded to 17% conversion of added substrate. This enhancement of the cytosolic 15-lipoxygenase activity, which was reversible by EGTA, was… CONTINUE READING