Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture.

@article{Boyce1983CalciumregulatedDO,
  title={Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture.},
  author={Steven T Boyce and Richard G. Ham},
  journal={The Journal of investigative dermatology},
  year={1983},
  volume={81 1 Suppl},
  pages={
          33s-40s
        }
}
An improved serum-free culture system has been developed for normal human epidermal keratinocytes (HK). Short-term clonal growth and differentiation studies are routinely performed in a defined medium consisting of optimized nutrient medium MCDB 153 supplemented with epidermal growth factor, insulin, hydrocortisone, ethanolamine, and phosphoethanolamine. A small amount of whole bovine pituitary extract (wBPE) is added for initiation of primary cultures, for frozen storage, and for serial… CONTINUE READING
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