Calcium phosphate transfection of primary hippocampal neurons.

@article{Sun2013CalciumPT,
  title={Calcium phosphate transfection of primary hippocampal neurons.},
  author={Miao Sun and Laura P. Bernard and Victoria L DiBona and Qian Wu and Huaye Zhang},
  journal={Journal of visualized experiments : JoVE},
  year={2013},
  volume={81},
  pages={
          e50808
        }
}
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells. 

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