Calcium phosphate precipitation in aqueous suspensions of phosphatidylserine-containing anionic liposomes

@article{Eanes2007CalciumPP,
  title={Calcium phosphate precipitation in aqueous suspensions of phosphatidylserine-containing anionic liposomes},
  author={E. Eanes and A. W. Hailer},
  journal={Calcified Tissue International},
  year={2007},
  volume={40},
  pages={43-48}
}
SummaryLiposomes prepared from 6.3∶1.8∶0.9∶ 1.0 molar mixtures of phosphatidylcholine, dicetyl phosphate, cholesterol, and phosphatidylserine, respectively, (PS(+) liposomes) were compared with similarly prepared liposomes without the phosphatidylserine (PS(−) liposomes) for their effect on calcium phosphate precipitate formation in aqueous solutions at pH 7.4 and 22°C. The liposomes, encapsulated with 50 mM phosphate (PI), were suspended in buffered 2.2 mM CaCl2, 0 or 1.5 mM KH2PO4 solutions… Expand
Modulation of calcium phosphate formation by phosphatidate-containing anionic liposomes
TLDR
Transmission electron microscopical analysis suggests that DOPA-containing lipid bilayers adhering to the seed crystals inhibited extraliposomal mineralization by encapsulating the crystals within the liposomes and/or by blocking potential growth sites on the crystal faces. Expand
An ultrastructural study of calcium phosphate formation in multilamellar liposome suspensions
TLDR
Transmission electron microscopical analysis showed that the liposome preparation consisted of multilamellar, multicompartmental vesicular structures that had many features in common with the sequence of mineral deposition occurring in matrix vesicle-mediated calcification. Expand
In vitro inhibition of membrane-mediated calcification by novel phosphonates
The effects of a series of novel phosphonates on the kinetics of mineral development in an ionophore-primed 7:2:1 phosphatidylcholine (PC): dicetylphosphate (DCP): cholesterol (Chol) liposomal modelExpand
The effect of phosphatidylserine onin vitro hydroxyapatite growth and proliferation
TLDR
The data suggest that PS may have multiple effects on hydroxyapatite formationin situ, and that mineral-PS interactions can retard crystal proliferation. Expand
Proteoliposomes in nanobiotechnology
TLDR
Current prospects of proteoliposomes for biotechnological applications are highlighted, including the construction of nanosensors and of a multi-protein nanovesicular biomimetic to study the processes of initiation of skeletal mineralization. Expand
Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells.
TLDR
It is suggested that PSL is able to stimulate dentin formation in dental pulps by being gradually incorporated into HDPC cytoplasm for several days. Expand
Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization.
TLDR
The potential use of proteoliposome systems as chondrocyte- and osteoblast-derived MVs biomimetics, as a means of reconstituting a phospholipid microenvironment in a manner that recapitulates the native functional MV microenvironment is discussed. Expand
Nature of phosphate substrate as a major determinant of mineral type formed in matrix vesicle-mediated in vitro mineralization: An FTIR imaging study.
TLDR
It is hypothesized that, at high PPi concentrations, PPi by itself or Pi released by partial PPi hydrolysis could act as inhibitors of alkaline phosphatase activity, thereby preventing complete hydrolytic of PPi to Pi, and thus resulting in the accumulation of calcium pyrophosphate dihydrate. Expand
Effects of phosphatidylserine coatings on titanium on inflammatory cells and cell-induced mineralisation in vitro.
TLDR
The high mineralisation potential of phosphatidylserine-coated titanium, together with the lower level of inflammatory response, supports the further development of this technology for coating osteointegrative devices. Expand
Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.
TLDR
The prospects of employing upcoming experimental models to deepen the understanding of MV-mediated mineralization and mineralization disorders such as the use of reconstituted lipid vesicles, proteoliposomes and, native sample preparations and high-resolution technologies are discussed. Expand
...
1
2
...

References

SHOWING 1-10 OF 33 REFERENCES
Liposome-Mediated calcium phosphate formation in metastable solutions
TLDR
The present findings suggest that when the external solution phase was metastable, the apparent coupling of large additional Ca2+ losses with intraliposomal precipitation was due to secondary precipitation brought about by the seeding action of intralIPosomal crystals penetrating into the internal solution. Expand
Calcium phosphate formation in aqueous suspensions of multilamellar liposomes
TLDR
The findings suggest that the precipitation within liposomes is similar to that which occurs in macro-volume synthetic systems but that the precipitated solid eventually impairs the integrity of the surrounding intermembranous space. Expand
Studies on the mechanism of membrane fusion: role of phosphate in promoting calcium ion induced fusion of phospholipid vesicles.
TLDR
Calcium and phosphate were shown to promote phospholipid phase separations and vesicle fusion under conditions in which calcium alone is ineffective, indicating that formation of PS calcium phosphate complexes may facilitate the molecular segregation of PS into distinct domains. Expand
Phosphatidate and oxidized fatty acids are calcium ionophores. Studies employing arsenazo III in liposomes.
TLDR
Data suggest that phosphatidic acid and oxidized di- and trienoic fatty acids, which act as calcium ionophores in model bilayers, could serve as "endogenous ionophore" in cells. Expand
Localization of phosphatidylserine in isolated chick epiphyseal cartilage matrix vesicles with trinitrobenzenesulfonate
TLDR
These findings add further evidence that binding of Ca by acidic phospholipids within the matrix vesicles may be involved in mineral deposition in epiphyseal cartilage. Expand
Effect of phospholipids on the transformation of amorphous calcium phosphate to hydroxyapatitein vitro
TLDR
The conversion of amorphous calcium phosphate to crystalline hydroxyapatite (HA) was studied in vitro in the presence or absence of phosphatidyl serine (PS) and other phospholipids, adding further evidence that lipids may play a role in the control of normal mineralization in vivo. Expand
Modulation of membrane fusion by membrane fluidity: temperature dependence of divalent cation induced fusion of phosphatidylserine vesicles.
We have investigated the temperature dependence of the fusion of phospholipid vesicles composed of pure bovine brain phosphatidylserine (PS) induced by Ca2+ or Mg2+. Aggregation of the vesicles wasExpand
The role of synthetic and bone extracted Ca-phospholipid-PO4 complexes in hydroxyapatite formation
TLDR
The evidence supports the possiblity that a similar complex is responsible for the initial mineralization in matrix vesicles, and suggests that the complex isolated from bone was formed in a manner similar to the complexes in the authors' in vitro experiments. Expand
Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
TLDR
The name cochleate lipid cylinders is suggested for the spiral structures formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders in phosphatidylserine preparations. Expand
Lipid composition of isolated epiphyseal cartilage cells, membranes and matrix vesicles.
  • R. Wuthier
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 1975
TLDR
Based on the fact that the matrix vesicles were significantly enriched in free cholesterol, sphingomyelin, glycolipids and serine-phosphoglycerides, it is concluded that they are derived from the plasma membrane of the cell, supporting earlier conclusions based upon morphological and enzymological evidence. Expand
...
1
2
3
4
...