Calcium ionophore polarizes ooplasmic segregation in ascidian eggs.

@article{Jeffery1982CalciumIP,
  title={Calcium ionophore polarizes ooplasmic segregation in ascidian eggs.},
  author={William R Jeffery},
  journal={Science},
  year={1982},
  volume={216 4545},
  pages={545-7}
}
Calcium ionophore A23187 promotes ooplasmic segregation and orange crescent formation in eggs of the ascidian Boltenia villosa. When eggs were exposed to a gradient A23187 the orange crescent was induced to form in the region corresponding to the highest concentration of ionophore. This result is consistent with the hypothesis that a local increase in intracellular calcium polarizes cytoplasmic localization in the ascidian embryo. 

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