Calcium influx but not pH or ATP level mediates glutamate-induced changes in intracellular magnesium in cortical neurons.

@article{Rajdev1995CalciumIB,
  title={Calcium influx but not pH or ATP level mediates glutamate-induced changes in intracellular magnesium in cortical neurons.},
  author={Sunita Rajdev and Ian J. Reynolds},
  journal={Journal of neurophysiology},
  year={1995},
  volume={74 3},
  pages={
          942-9
        }
}
1. We have recently shown that glutamate increases [Mg2+]i in cultured rat cortical neurons. However, the mechanism of this increase in [Mg2+]i is not well understood. We used fluorescence microscopic methods to measure [Mg2+]i, [Ca2+]i, and pHi in single neurons. Intracellular ATP analysis was performed by high-performance liquid chromatography (HPLC). 2. A 25-mM NH4Cl pulse followed by Na(+)-free wash rapidly acidified the cytosol. In 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF… CONTINUE READING
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