Calcium-induced alterations in mitochondrial morphology quantified in situ with optical scatter imaging.

@article{Boustany2002CalciuminducedAI,
  title={Calcium-induced alterations in mitochondrial morphology quantified in situ with optical scatter imaging.},
  author={Nada N. Boustany and Rebekah A. Drezek and Nitish V. Thakor},
  journal={Biophysical journal},
  year={2002},
  volume={83 3},
  pages={1691-700}
}
Optical scatter imaging (OSI), a technique we developed recently, was used to measure the ratio of wide-to-narrow angle scatter (OSIR) within endothelial cells subjected to calcium overload (1.6 mM) after permeabilization by ionomycin. Within a few minutes of calcium overload, the mitochondria, which started as elongated organelles, rounded up into spherically shaped particles. This change in morphology was accompanied by a statistically significant 14% increase in OSIR in the cells' cytoplasm… CONTINUE READING