Calcium-independent subtilisin by design.

  title={Calcium-independent subtilisin by design.},
  author={Travis Gallagher and Peter Bryan and Gary L Gilliland},
  volume={16 2},
A version of subtilisin BPN' lacking the high affinity calcium site (site A) has been produced through genetic engineering methods, and its crystal structure refined at 1.8 A resolution. This protein and the corresponding version containing the calcium A site are described and compared. The deletion of residues 75-83 was made in the context of four site-specific replacements previously shown to stabilize subtilisin. The helix that in wild type is interrupted by the calcium binding loop, is… CONTINUE READING
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