Calcium-independent cell volume regulation in human lymphocytes. Inhibition by charybdotoxin

@article{Grinstein1990CalciumindependentCV,
  title={Calcium-independent cell volume regulation in human lymphocytes. Inhibition by charybdotoxin},
  author={Sergio Grinstein and Joshua D. Smith},
  journal={The Journal of General Physiology},
  year={1990},
  volume={95},
  pages={97 - 120}
}
The properties of the K+ pathway underlying regulatory volume decrease (RVD) in human blood lymphocytes were investigated. Evidence is presented for the existence of three types of K+ conductance in these cells. Ionomycin, a Ca2+ ionophore, induced a K(+)-dependent hyperpolarization, indicating the presence of Ca2(+)-activated K+ channels, which were blocked by charybdotoxin (CTX). CTX also induced a depolarization of the resting membrane potential, even at subphysiological cytosolic [Ca2… CONTINUE READING