Calcium imaging of motoneuron activity in the en-bloc spinal cord preparation of the neonatal rat.

@article{LevTov1995CalciumIO,
  title={Calcium imaging of motoneuron activity in the en-bloc spinal cord preparation of the neonatal rat.},
  author={Aharon Lev-Tov and Michael J. O'Donovan},
  journal={Journal of neurophysiology},
  year={1995},
  volume={74 3},
  pages={1324-34}
}
1. This paper describes the use of calcium imaging to monitor patterns of activity in neonatal rat motoneurons retrogradely labeled with the calcium-sensitive dye, calcium green-dextran. 2. Pressure ejection of calcium green-dextran into ventral roots and into the surgically peeled ventrolateral funiculi (VLF) at the lumbar cord labeled spinal motoneurons and interneurons. The back labeled motoneurons often formed two or three discrete clusters of cells. 3. Fluorescent changes (10-20%) could be… CONTINUE READING

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