Calcium-driven DNA synthesis by a high-fidelity DNA polymerase

@inproceedings{Ralec2017CalciumdrivenDS,
  title={Calcium-driven DNA synthesis by a high-fidelity DNA polymerase},
  author={Céline Ralec and Etienne Henry and Mélanie Lemor and Tom Killelea and Ghislaine Henneke},
  booktitle={Nucleic acids research},
  year={2017}
}
Divalent metal ions, usually Mg2+, are required for both DNA synthesis and proofreading functions by DNA polymerases (DNA Pol). Although used as a non-reactive cofactor substitute for binding and crystallographic studies, Ca2+ supports DNA polymerization by only one DNA Pol, Dpo4. Here, we explore whether Ca2+-driven catalysis might apply to high-fidelity (HiFi) family B DNA Pols. The consequences of replacing Mg2+ by Ca2+ on base pairing at the polymerase active site as well as the editing of… CONTINUE READING
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