Calcium-dependent regulation of the caldesmon-heavy meromyosin interaction by caltropin.

  title={Calcium-dependent regulation of the caldesmon-heavy meromyosin interaction by caltropin.},
  author={Rajam S. Mani and Cyril M. Kay},
  volume={32 41},
The binding of chicken gizzard caldesmon to smooth muscle heavy meromyosin (HMM) was studied using caldesmon-Sepharose 4B affinity chromatography, far-ultraviolet circular dichroism (CD), and the fluorescent probe acrylodan. When HMM was applied to a caldesmon-Sepharose column in the presence of 40 mM NaCl, most of the protein was retained on the column, and HMM could be eluted by increasing the NaCl level to 0.5 M; this interaction was not Ca(2+)-dependent. Far-UV CD studies indicated an… CONTINUE READING


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