Calcium binding by alpha-lactalbumin in human milk and bovine milk.

  title={Calcium binding by alpha-lactalbumin in human milk and bovine milk.},
  author={B O L{\"o}nnerdal and Carol E Glazier},
  journal={The Journal of nutrition},
  volume={115 9},
The metal-binding property of alpha-lactalbumin (alpha-LA) in human milk was studied and compared to that of bovine milk alpha-LA. Gel filtration on Sephadex G-75 at physiological pH and ionic strength separated alpha-LA in human and bovine milk from most other proteins. The only metal ion associated with alpha-LA under these conditions was Ca2+. Minor protein contaminants were removed by ion-exchange chromatography, and the ratio of Ca2+:alpha-LA was determined in the isolated protein… CONTINUE READING


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