Corpus ID: 18371554

Calcium Alginate: A Support Material for Immobilization of Proteases from Newly Isolated Strain of Bacillus subtilis KIBGE-HAS

  title={Calcium Alginate: A Support Material for Immobilization of Proteases from Newly Isolated Strain of Bacillus subtilis KIBGE-HAS},
  author={Abida Anwar and Shah Ali Ul Qader and A Raiz and Samina Iqbal and Abid Azhar},
Partially purified neutral protease was entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of the enzyme showed no changes before and after immobilization and remained stable at 50°C and 7.5 respectively. However substrate concentration for maximum enzyme activity of immobilized enzyme was shifted from 0.45% to 0.8%. Reaction time for immobilized enzyme assay was also increased by 5 minutes with reference to soluble enzyme i.e., from 15… Expand

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