Corpus ID: 18371554

Calcium Alginate: A Support Material for Immobilization of Proteases from Newly Isolated Strain of Bacillus subtilis KIBGE-HAS

@inproceedings{Anwar2009CalciumAA,
  title={Calcium Alginate: A Support Material for Immobilization of Proteases from Newly Isolated Strain of Bacillus subtilis KIBGE-HAS},
  author={Abida Anwar and Shah Ali Ul Qader and A Raiz and Samina Iqbal and Abid Azhar},
  year={2009}
}
Partially purified neutral protease was entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of the enzyme showed no changes before and after immobilization and remained stable at 50°C and 7.5 respectively. However substrate concentration for maximum enzyme activity of immobilized enzyme was shifted from 0.45% to 0.8%. Reaction time for immobilized enzyme assay was also increased by 5 minutes with reference to soluble enzyme i.e., from 15… Expand

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References

SHOWING 1-10 OF 24 REFERENCES
Characterization of dextransucrase immobilized on calcium alginate beads from Leuconostoc mesenteroides PCSIR-4.
TLDR
Immobilization of dextransucrase from Leuconostoc mesenteroides PCSIR-4 on alginate is optimized for application in the production ofdextran from sucrose and maximum stability of immobilized dextran was achieved at 25 degrees C with respect to time. Expand
Purification, characterization and immobilization of a keratinase from Aspergillus oryzae
Abstract A keratinase enzyme was isolated and purified from a feather-degrading culture of Aspergillus oryzae . Fractional precipitation of the crude enzyme with ethanol, acetone and ammonium sulfateExpand
THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN
  • M. L. Anson
  • Chemistry, Medicine
  • The Journal of general physiology
  • 1938
TLDR
It is considered simpler and more useful to describe completely the procedures as they are now used in this laboratory, and to avoid confusion about results already published no radical changes have been made. Expand
ON TYROSINE AND TRYPTOPHANE DETERMINATIONS IN PROTEINS
The article summarizes research into the existing methods for the quantitative determination of tyrosine and tryptophane in proteins. Limitations to the accuracy of the Folin-Looney method (reactionExpand
On the Internet
On tyrosine and traditional Aztec alcoholic beverage
  • Ciocalteu,
  • 1929
Series
  • Series
  • 2006
Review , Enzyme immobilization , and Mr . M . Hussain from The Karachi Institute of the state of art in Biotechnology , Iranian J . Biotechnology and Genetic Engineering for the Biotechnol
  • 2003
Ghatge and strain of Aspergillus Oryzae AWT 20 and its V.V
  • beads. The Internet J. Microbiol.,
  • 1998
Entrapment of in alginate beads : Single step protocol for enzymes and cells in calcium alginate . In purification and thermal stabilization
  • Process Immobilisation of Enzymes and Cells , Humana Press , Biochem .
  • 1997
...
1
2
3
...