Calbindin-D28k is a more reliable marker of human Purkinje cells than standard Nissl stains: A stereological experiment

  title={Calbindin-D28k is a more reliable marker of human Purkinje cells than standard Nissl stains: A stereological experiment},
  author={E. R. Whitney and T. L. Kemper and D. Rosene and M. Bauman and G. Blatt},
  journal={Journal of Neuroscience Methods},
  • E. R. Whitney, T. L. Kemper, +2 authors G. Blatt
  • Published 2008
  • Biology, Medicine
  • Journal of Neuroscience Methods
  • In a study of human Purkinje cell (PC) number, a striking mismatch between the number of PCs observed with the Nissl stain and the number of PCs immunopositive for calbindin-D28k (CB) was identified in 2 of the 10 brains examined. In the remaining eight brains this mismatch was not observed. Further, in these eight brains, analysis of CB immunostained sections counterstained with the Nissl stain revealed that more than 99% Nissl stained PCs were also immunopositive for CB. In contrast, in the… CONTINUE READING
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