Caged RNA: photo-control of a ribozyme reaction

  title={Caged RNA: photo-control of a ribozyme reaction},
  author={Steven G. Chaulk and Andrew M. MacMillan},
  journal={Nucleic acids research},
  volume={26 13},
We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and… CONTINUE READING

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  • J B Murray, D P Terwey, +4 authors W G Scott
  • Cell
  • 1998


  • Brubaker, M J Dyer, D H Stoddard, B Koshland
  • Biochemistry
  • 1996


  • J H Cate, A R Gooding, +5 authors J A Doudna
  • Science
  • 1996

Chem. Biol

  • C R Allerson, G L Verdine
  • Chem. Biol
  • 1995


  • S T Sigurdsson, T Tuschl, F Eckstein
  • RNA
  • 1995

Curr. Opin. Struct. Biol.J.R. Science

  • A M Pyle, J B Green
  • Curr. Opin. Struct. Biol.J.R. Science
  • 1994

Genes Dev

  • A M Macmillan, C C Query, C R Allerson, S Chen, G L Verdine, P A Sharp
  • Genes Dev
  • 1994

Nucleic Acids Res

  • D C Capaldi, C B Reese
  • Nucleic Acids Res
  • 1994

Tetrahedron Lett

  • J T Goodwin, G D Glick
  • Tetrahedron Lett
  • 1994

Annu. Rev. Physiol

  • R Y Tsien
  • Annu. Rev. Physiol
  • 1993

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