Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC.

@article{Jang2002CaffeineIA,
  title={Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC.},
  author={Myoungsouk. Jang and Min-Chul Shin and Il Koo Kang and Hyung-Hwan Baik and Yong-Ho Cho and Ju Chu and Eyong-B. Kim and Changyeoung Kim},
  journal={Journal of Korean Medical Science},
  year={2002},
  volume={17},
  pages={674 - 678}
}
Caffeine is one of the most widely consumed neuroactive drugs, coming mostly from everyday beverages such as coffee and tea. To investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, DNA fragmentation assay, and caspase-3 enzyme assay… CONTINUE READING

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