Caffeine- and ryanodine-sensitive Ca2+ stores of canine cerebrum and cerebellum neurons.

@article{Mszros1991CaffeineAR,
  title={Caffeine- and ryanodine-sensitive Ca2+ stores of canine cerebrum and cerebellum neurons.},
  author={L{\'a}szl{\'o} G. M{\'e}sz{\'a}ros and Pompeo Volpe},
  journal={The American journal of physiology},
  year={1991},
  volume={261 6 Pt 1},
  pages={C1048-54}
}
[3H]ryanodine binding to and Ca2+ release from microsomal fractions derived from canine cerebrum (CBR) and cerebellum (CBL) were investigated. High-affinity ryanodine binding sites were detected in both cerebrum and cerebellum microsomes [CBR: maximal binding capacity (Bmax) = 446 fmol/mg protein, dissociation constant (Kd) = 9 nM, Hill coefficient (n) = 0.95; CBL: Bmax = 650, Kd = 12, n = 1.8]. Ryanodine binding in both fractions was increased by millimolar concentrations of ATP [or its… CONTINUE READING