Caffeine-Induced Ca2+ Transients and Exocytosis in Paramecium Cells. A Correlated Ca2+ Imaging and Quenched-Flow/Freeze-Fracture Analysis

@article{Klauke1998CaffeineInducedCT,
  title={Caffeine-Induced Ca2+ Transients and Exocytosis in Paramecium Cells. A Correlated Ca2+ Imaging and Quenched-Flow/Freeze-Fracture Analysis},
  author={Norbert Klauke and Helmut Plattner},
  journal={The Journal of Membrane Biology},
  year={1998},
  volume={161},
  pages={65-81}
}
Caffeine causes a [Ca2+] i increase in the cortex of Paramecium cells, followed by spillover with considerable attenuation, into central cell regions. From [Ca2+]rest i ∼50 to 80 nm, [Ca2+]act i rises within ≤3 sec to 500 (trichocyst-free strain tl) or 220 nm (nondischarge strain nd9–28°C) in the cortex. Rapid confocal analysis of wildtype cells (7S) showed only a 2-fold cortical increase within 2 sec, accompanied by trichocyst exocytosis and a central Ca2+ spread during the subsequent ≥2 sec… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 83 references

Functional reconstitution of ion channels fromParameciumcortex into artificial liposomes.J

X. L. Zhou, C.W.M. Chan, Y. Siami, C. Kung
Membrane Biol.144:199–208 • 1995
View 8 Excerpts
Highly Influenced

Synchronization of different steps of the secretory cycle

H. Plattner, G. Knoll, R. Pape
Eur. J. Cell • 1993
View 3 Excerpts
Highly Influenced

Genetic dissection of the morphogenesis of exocytosis sites in Paramecium

M. Pouphile, M. Lefort-Tran, H. Plattner, M. Rossignol, J. Beisson
Biol. Cell • 1986
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…