Caenorhabditis elegans has two genes encoding functional d-aspartate oxidases.

  title={Caenorhabditis elegans has two genes encoding functional d-aspartate oxidases.},
  author={Masumi Katane and Yousuke Seida and Masae Sekine and Takemitsu Furuchi and Hiroshi Homma},
  journal={The FEBS journal},
  volume={274 1},
Four cDNA clones that were annotated in the database as encoding d-amino acid oxidase (DAAO) or d-aspartate oxidase (DASPO) were isolated by RT-PCR from Caenorhabditis elegans RNA. The proteins (Y69Ap, C47Ap, F18Ep, and F20Hp) encoded by the cloned cDNAs were expressed in Escherichia coli as recombinant proteins with an N-terminal His-tag. All proteins except F20Hp were recovered in the soluble fractions. The recombinant Y69Ap has functional DAAO activity, as it can deaminate neutral and basic… CONTINUE READING

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