Ca2+ transport in mitochondria from yeast expressing recombinant aequorin.

@article{Jung2004Ca2TI,
  title={Ca2+ transport in mitochondria from yeast expressing recombinant aequorin.},
  author={Dennis W Jung and Patrick C Bradshaw and Monica L Litsky and Douglas R. Pfeiffer},
  journal={Analytical biochemistry},
  year={2004},
  volume={324 2},
  pages={258-68}
}
We have expressed aequorin in mitochondria of the yeast Saccharomyces cerevisiae and characterized the resulting strain with respect to mitochondrial Ca(2+) transport in vivo and in vitro. When intact cells are suspended in water containing 1.4 mM ethanol and 14 mM CaCl(2), the matrix free Ca(2+) concentration is 200 nM, similar to the values expected in cytoplasm. Addition of ionophore ETH 129 allows an active accumulation of Ca(2+) and promptly increases the value to 1.2 microM. Elevated Ca(2… CONTINUE READING