Ca2+ mobilization and Cl efflux in submandibular salivary cells of adult and newborn rats.

@article{Martinez1989Ca2MA,
  title={Ca2+ mobilization and Cl efflux in submandibular salivary cells of adult and newborn rats.},
  author={J. Ricardo Martinez and Jean M. Camden},
  journal={Archives of oral biology},
  year={1989},
  volume={34 3},
  pages={147-52}
}
These cells were isolated by enzymatic digestion from glands of 1-day-old and fully mature rats. The effects of exposure to acetylcholine on cytosolic Ca2+ and Cl efflux were studied by, respectively, spectrofluorimetry of fura-2 and the net efflux of the isotopic tracer 36Cl from preloaded cells. In both types of cells, when incubated in Ca2+-containing solutions, acetylcholine initially caused a rapid, significant increase in cytosolic Ca2+ (from approx. 90 to 480-570 nmol), followed by a… CONTINUE READING