Ca2+ binding to phosphatidylcholine bilayers as studied by deuterium magnetic resonance. Evidence for the formation of a Ca2+ complex with two phospholipid molecules.

@article{Altenbach1984Ca2BT,
  title={Ca2+ binding to phosphatidylcholine bilayers as studied by deuterium magnetic resonance. Evidence for the formation of a Ca2+ complex with two phospholipid molecules.},
  author={Christian Altenbach and Joachim Seelig},
  journal={Biochemistry},
  year={1984},
  volume={23 17},
  pages={3913-20}
}
The binding of Ca2+ to bilayer membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was investigated with atomic absorption spectroscopy and deuterium magnetic resonance, leading to the following conclusions. Atomic absorption spectroscopy allowed the determination of the amount of Ca2+ bound to the membrane surface (Cb) at low Ca2+ concentrations (3-100 mM). Simultaneous measurements of the deuterium magnetic resonance spectra of POPC with specifically deuterated… CONTINUE READING

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