Ca2+ Uncaging in Nerve Terminals: A Three-Point Calibration Procedure.

@article{Kochubey2015Ca2UI,
  title={Ca2+ Uncaging in Nerve Terminals: A Three-Point Calibration Procedure.},
  author={Olexiy Kochubey and Ralf Schneggenburger},
  journal={Cold Spring Harbor protocols},
  year={2015},
  volume={2015 8},
  pages={
          761-8
        }
}
Ca(2+) uncaging can be used to create a spatially homogenous elevation of the intracellular free Ca(2+) concentration, [Ca(2+)]i, in cells. When applied to nerve terminals or secretory cells, this technique allows one to elicit transmitter release with a [Ca(2+)]i signal of measurable amplitude, and therefore to directly relate the rate of transmitter release to the measured [Ca(2+)]i. When combined with patch-clamp measurements, Ca(2+) uncaging is done by introducing a Ca(2+)-loaded… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 19 REFERENCES

Quantitative aspects of calcium fluorimetry.

  • Cold Spring Harbor protocols
  • 2013
VIEW 1 EXCERPT

Calibration of fluorescent calcium indicators.

  • Cold Spring Harbor protocols
  • 2011
VIEW 2 EXCERPTS