Ca2+/protein modulator-dependent and -independent cyclic GMP phosphodiesterase from hog heart.

@article{Nasu1978Ca2proteinMA,
  title={Ca2+/protein modulator-dependent and -independent cyclic GMP phosphodiesterase from hog heart.},
  author={S Nasu and K Shioji and I Ueda and Yoshinori Tanigawa and Makoto Shimoyama},
  journal={Journal of biochemistry},
  year={1978},
  volume={83 5},
  pages={
          1449-58
        }
}
Ca2+/protein modulator-dependent and -independent guanosine 3':5'-monophosphate (cGMP) phosphodiesterases were separated from hog heart. The protein modulator-free Ca2+/protein modulator-dependent enzyme was partially purified by repeated DEAE-cellulose column chromatography and heat treatment. The final preparation of this enzyme showed no significant basal activity under the standard assay conditions. Lineweaver-Burk plots of the Ca2+/protein modulator-dependent enzyme activity indicated the… 
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