Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization.

Abstract

Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) has been purified from hen whole brain. The enzyme was purified 3000-fold using phosphocellulose and calmodulin-Agarose column chromatography. The specific activity was 200 nmol/min/mg protein. Microtubule associated protein-2 (MAP-2) was used as a substrate to assess the activity of the enzyme… (More)

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@article{Gupta1992Ca2calmodulindependentPK, title={Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization.}, author={R. P. Gupta and Dan Lapadula and Mohammed B Abou-Donia}, journal={Biochemical pharmacology}, year={1992}, volume={43 9}, pages={1975-88} }