Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization.

@article{Gupta1992Ca2calmodulindependentPK,
  title={Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization.},
  author={Ram Prakash Gupta and D M Lapadula and Mohamed Bahie Abou-Donia},
  journal={Biochemical pharmacology},
  year={1992},
  volume={43 9},
  pages={1975-88}
}
Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) has been purified from hen whole brain. The enzyme was purified 3000-fold using phosphocellulose and calmodulin-Agarose column chromatography. The specific activity was 200 nmol/min/mg protein. Microtubule associated protein-2 (MAP-2) was used as a substrate to assess the activity of the enzyme during purification and for its characterization. CaM-kinase II consisted of alpha and beta/beta' subunits of molecular weights 46,000 and 55… CONTINUE READING

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