Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes.

@article{Santiago2010CaSD,
  title={Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes.},
  author={Demetrio J Santiago and Jerald W Curran and Donald M. Bers and W. Jonathan Lederer and Michael D. Stern and Eduardo R{\'i}os and T Randolph Shannon},
  journal={Biophysical journal},
  year={2010},
  volume={98 10},
  pages={2111-20}
}
Diastolic Ca leak from the sarcoplasmic reticulum (SR) of ventricular myocytes reduces the SR Ca content, stabilizing the activity of the SR Ca release channel ryanodine receptor for the next beat. SR Ca leak has been visualized globally using whole-cell fluorescence, or locally using confocal microscopy, but never both ways. When using confocal microscopy, leak is imaged as "Ca sparks," which are fluorescent objects generated by the local reaction-diffusion of released Ca and cytosolic… CONTINUE READING