Ca(2+) monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor.


Calcium (Ca(2+))-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10 μm) measurement of intracellular Ca(2+) in Plasmodium is technically challenging, and thus Ca(2+) regulation in this human pathogen is not well understood. Here we analyze Ca(2+) homeostasis via a new approach using transgenic P. falciparum expressing the Ca(2+) sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca(2+) concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30 nM), and is increased in the other blood stages (>300 nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca(2+) level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca(2+) signaling in P. falciparum and is applicable for drug screening.

DOI: 10.1038/srep23454

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@article{Pandey2016Ca2MI, title={Ca(2+) monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor.}, author={Kishor Pandey and Pedro E Ferreira and Takeshi Ishikawa and Takeharu Nagai and Osamu Kaneko and Kazuhide Yahata}, journal={Scientific reports}, year={2016}, volume={6}, pages={23454} }