Ca++-transport across basal-lateral plasma membranes from rat small intestinal epithelial cells


Basal-lateral plasma membrane vesicles were isolated from rat duodenum and jejunum by a Percoll gradient centrifugation technique. Ca-uptake into and Ca-release from the vesicles was studied by a rapid filtration method. In the absence of Na (K-medium) at a Ca concentration of 0.05 mmol/liter and pH 7.4, addition of 5mm MgATP stimulated Ca-uptake up to 10… (More)
DOI: 10.1007/BF01870469


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