Ca(2+)-related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay.

@article{Dasgupta1993Ca2relatedCI,
  title={Ca(2+)-related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay.},
  author={Siddhartha Dasgupta and Clytee L Mills and Lynn R Fraser},
  journal={Journal of reproduction and fertility},
  year={1993},
  volume={99 1},
  pages={135-43}
}
Chlortetracycline (CTC) fluorescence patterns were used to assess Ca(2+)-related changes in the capacitation state of human spermatozoa incubated under conditions that would affect their intracellular Ca2+ levels. Initial experiments were designed to identify consistently occurring patterns and to correlate these with acrosomal status. Incubation for up to 1 h with the ionophore A23187 (10 mumol l-1), known to promote capacitation and acrosomal exocytosis, allowed the identification of three… CONTINUE READING
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