Ca(2+)-dependent interactions between Gla and EGF domains in human coagulation factor IX.

@article{Medved1994Ca2dependentIB,
  title={Ca(2+)-dependent interactions between Gla and EGF domains in human coagulation factor IX.},
  author={Leonid Medved and A Vysotchin and Kenneth C Ingham},
  journal={Biochemistry},
  year={1994},
  volume={33 2},
  pages={478-85}
}
The Ca(2+)-induced interaction between the Gla and EGF domains of human factor IX was investigated by means of three fragments: 6-kDa Gla, 19-kDa (EGF)2, and 25-kDa Gla-(EGF)2. Size-exclusion chromatography and spectroscopic measurements revealed that the Gla-EGF interaction is rather strong; it can be reconstituted by mixing the 6-kDa and 19-kDa fragments… CONTINUE READING