Ca(2+)-dependent and Ca(2+)-independent NO-synthesizing activities of human primordial placenta.

In order to localize the site of production of nitrogen monoxide (NO) in first trimester human pregnancy, the cytosol and microsome fractions prepared from homogenized primordial placentas were tested for NO-synthase (NOS) activities by measuring the NADPH-dependent conversion of [3H]arginine to [3H]citrulline. Our results demonstrate that Ca(2+)-dependent… CONTINUE READING