CYP3A4 Activity in the Presence of Organic Cosolvents, Ionic Liquids, or Water‐Immiscible Organic Solvents

@article{Chefson2007CYP3A4AI,
  title={CYP3A4 Activity in the Presence of Organic Cosolvents, Ionic Liquids, or Water‐Immiscible Organic Solvents},
  author={Amandine Chefson and Karine Auclair},
  journal={ChemBioChem},
  year={2007},
  volume={8}
}
P450 enzymes have attracted the attention of chemists for decades because of their impressive ability to catalyze the hydroxylation of inactivated CH bonds. However, their use for synthesis in aqueous systems is limited. We report here a survey of the activity of purified human CYP3A4 in the presence of organic solvents or ionic liquids. We show that CYP3A4 tolerates only small amounts (<15 %) of water‐miscible organic cosolvents or ionic liquids before its activity toward testosterone drops… Expand
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