CYP2E1 hydroxylation of aniline involves negative cooperativity.

  title={CYP2E1 hydroxylation of aniline involves negative cooperativity.},
  author={Jessica H Hartman and Katie Knott and Grover P. Miller},
  journal={Biochemical pharmacology},
  volume={87 3},
CYP2E1 plays a role in the metabolic activation and elimination of aniline, yet there are conflicting reports on its mechanism of action, and hence relevance, in aniline metabolism. Based on our work with similar compounds, we hypothesized that aniline binds two CYP2E1 sites during metabolism resulting in cooperative reaction kinetics and tested this hypothesis through rigorous in vitro studies. The kinetic profile for recombinant CYP2E1 demonstrated significant negative cooperativity based on… CONTINUE READING


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View 4 Excerpts
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CYP2A6: genetics, structure, regulation, and function.

Drug metabolism and drug interactions • 2012
View 1 Excerpt

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Drug metabolism and disposition: the biological fate of chemicals • 2012
View 4 Excerpts

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