CRM1-dependent trafficking of retroviral Gag proteins revisited.

  title={CRM1-dependent trafficking of retroviral Gag proteins revisited.},
  author={Mariju F Baluyot and Sarah A Grosse and Terri D. Lyddon and Sanath Kumar Janaka and Marc C. Johnson},
  journal={Journal of virology},
  volume={86 8},
We analyzed the nuclear trafficking ability of Gag proteins from six retroviral genera. Contrary to a previous report, human immunodeficiency virus type 1 (HIV-1) Gag showed no propensity to cycle through the nucleus. The only Gag protein that displayed CRM1-dependent nuclear cycling was that of Rous sarcoma virus (RSV). Surprisingly, this cycling could be eliminated without compromising infectivity by replacing the RSV Gag N-terminal matrix (MA) domain with HIV MA.