CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination.

@article{Dewar2017CRL2Lrr1PU,
  title={CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination.},
  author={James M. Dewar and Emily Low and Matthias Mann and Markus R{\"a}schle and Johannes C. Walter},
  journal={Genes & development},
  year={2017},
  volume={31 3},
  pages={275-290}
}
A key event during eukaryotic replication termination is the removal of the CMG helicase from chromatin. CMG unloading involves ubiquitylation of its Mcm7 subunit and the action of the p97 ATPase. Using a proteomic screen in Xenopus egg extracts, we identified factors that are enriched on chromatin when CMG unloading is blocked. This approach identified the E3 ubiquitin ligase CRL2Lrr1, a specific p97 complex, other potential regulators of termination, and many replisome components. We show… CONTINUE READING
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