CRISPRing into the woods

@inproceedings{Tsai2015CRISPRingIT,
  title={CRISPRing into the woods},
  author={Chung-Jui Tsai and Liang-Jiao Xue},
  booktitle={GM crops & food},
  year={2015}
}
The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application of CRISPR/Cas9 for biallelic mutations in stably transformed Populus, extending the species range of this powerful technology to woody perennials. An underappreciated obstacle in genome editing of outcrossing species is the frequent occurrence of sequence polymorphisms that can render CRISPR/Cas9 unproductive… CONTINUE READING
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