CRISPR genome engineering and viral gene delivery: A case of mutual attraction

@article{Schmidt2015CRISPRGE,
  title={CRISPR genome engineering and viral gene delivery: A case of mutual attraction},
  author={Florian Schmidt and Dirk Grimm},
  journal={Biotechnology Journal},
  year={2015},
  volume={10}
}
The adaptation of the CRISPR/Cas9 DNA engineering machinery for mammalian cells has revolutionized our approaches to low‐ or high‐throughput genome annotation and paved the way for conceptually novel therapeutic strategies. A large part of the attraction of CRISPR stems from the small size of its two core components – Cas9 and gRNA – and hence its compatibility with virtually any available viral vector delivery system. As a result, over the past two years, four major classes of viral vectors… 

REVIEW genome editing in animals using AAV-CRISPR system : In vivo applications to translational research of human disease

  • Biology, Engineering
  • 2019
How the combined use of small vivo Cas9 orthologues, tissue-specific minimal promoters, AAV serotypes, and different routes of administration has advanced the development of efficient and precise genome editing is discussed and comprehensively review the various in vivo AAV-CRISPR systems that have been effectively used in animals.

In vivo genome editing in animals using AAV-CRISPR system: applications to translational research of human disease

How the combined use of small Cas9 orthologues, tissue-specific minimal promoters, AAV serotypes, and different routes of administration has advanced the development of efficient and precise in vivo genome editing is discussed and comprehensively review the various AAV-CRISPR systems that have been effectively used in animals.

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Cationic Polymer-Mediated CRISPR/Cas9 Plasmid Delivery for Genome Editing.

This study defines a new strategy for the delivery of the large plasmid encoding Cas9/sgRNA for efficient genome editing, which results in efficient editing at two genome loci, namely, hemoglobin subunit beta and rhomboid 5 homolog 1.

Appropriate Delivery of the CRISPR/Cas9 System through the Nonlysosomal Route: Application for Therapeutic Gene Editing

Given their high biosafety, PAR‐Lipos are used to mediate the knockout of the oncogene CDC6 in vivo, which results in significant tumor growth inhibition, and may provide a useful reference for enhancing the delivery of gene editing systems, thus improving the potential for their future clinical applications.
...

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