CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

@article{Barrangou2007CRISPRPA,
  title={CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes},
  author={R. Barrangou and C. Fremaux and H. Deveau and M. Richards and P. Boyaval and S. Moineau and D. Romero and P. Horvath},
  journal={Science},
  year={2007},
  volume={315},
  pages={1709 - 1712}
}
  • R. Barrangou, C. Fremaux, +5 authors P. Horvath
  • Published 2007
  • Medicine, Biology
  • Science
  • Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and… CONTINUE READING

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    Information on materials and methods for the generation of phage-resistant mutants, engineering of CRISPR spacers (Figs. S4 and S5), and inactivation of cas genes is available on Science Online

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    Materials and methods are available as supporting material on Science Online