CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation

  title={CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation},
  author={Xin Han and Zongbin Liu and Myeong Chan Jo and Kai Zhang and Ying Li and Zihua Zeng and Nan Li and Youli Zu and Lidong Qin},
  booktitle={Science Advances},
The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) nuclease system represents an efficient tool for genome editing and gene function analysis. It consists of two components: single-guide RNA (sgRNA) and the enzyme Cas9. Typical sgRNA and Cas9 intracellular delivery techniques are limited by their reliance on cell type and exogenous materials as well as their toxic effects on cells (for example, electroporation). We introduce and optimize a… CONTINUE READING
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