CRISPR-Cas System: History and Prospects as a Genome Editing Tool in Microorganisms

  title={CRISPR-Cas System: History and Prospects as a Genome Editing Tool in Microorganisms},
  author={Muhammad Rizwan Javed and Maria Sadaf and Temoor Ahmed and Amna Jamil and Marium Nawaz and Hira Fatima Abbas and Anam Ijaz},
  journal={Current Microbiology},
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR or more precisely CRISPR-Cas) system has proven to be a highly efficient and simple tool for achieving site-specific genome modifications in comparison to Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs). The discovery of bacterial defense system that uses RNA-guided DNA cleaving enzymes for producing double-strand breaks along CRISPR has provided an exciting alternative to ZFNs and TALENs… 

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