CRISPR-Cas System: History and Prospects as a Genome Editing Tool in Microorganisms

@article{Javed2018CRISPRCasSH,
  title={CRISPR-Cas System: History and Prospects as a Genome Editing Tool in Microorganisms},
  author={Muhammad Rizwan Javed and Maria Sadaf and Temoor Ahmed and Amna Jamil and Marium Nawaz and Hira Fatima Abbas and Anam Ijaz},
  journal={Current Microbiology},
  year={2018},
  volume={75},
  pages={1675-1683}
}
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR or more precisely CRISPR-Cas) system has proven to be a highly efficient and simple tool for achieving site-specific genome modifications in comparison to Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs). The discovery of bacterial defense system that uses RNA-guided DNA cleaving enzymes for producing double-strand breaks along CRISPR has provided an exciting alternative to ZFNs and TALENs… 

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References

SHOWING 1-10 OF 62 REFERENCES

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

TLDR
The use of type II bacterial CRISPR-Cas system in Saccharomyces cerevisiae for genome engineering provides foundations for a simple and powerful genome engineering tool for site-specific mutagenesis and allelic replacement in yeast.

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

TLDR
It is envisioned that CRISPR–Cas genome editing has the potential to change the landscape of genome editing in lactic acid bacteria, and other Gram-positive bacteria.

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

TLDR
The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases.

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

TLDR
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.

CRISPR/Cas technology: a revolutionary approach for genome engineering

TLDR
A new type of site-specific nu-cleases, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, has emerged and raised a revolution in the genomics field.

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

TLDR
It is demonstrated that the Cas9–crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system introduces in vitro a double-strand break at a specific site in DNA containing a sequence complementary to crRNA, paving the way for engineering of universal programmable RNA-guided DNA endonucleases.

Gene silencing by CRISPR interference in mycobacteria.

TLDR
It is shown that co-expression of the codon-optimized dCas9 of S. pyogenes with sequence-specific guide RNA results in complete repression of individual or multiple targets in mycobacteria, and CRISPRi thus offers a simple, rapid and cost-effective tool for selective control of gene expression in myCobacteria.

CRISPR/Cas, the Immune System of Bacteria and Archaea

TLDR
Clustered regularly interspaced short palindromic repeats (CRISPR) form peculiar genetic loci, which provide acquired immunity against viruses and plasmids by targeting nucleic acid in a sequence-specific manner.

CRISPR-Cas systems for editing, regulating and targeting genomes

TLDR
A modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells, which will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.
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