Corpus ID: 198135507

CRISPR-Cas IIIA Csm 6 CA RF Domain Is a Ring Nuclease Triggering Stepwise cA 4 Cleavage with ApA > p Formation Terminating RNase Activity Graphical

@inproceedings{Jia2019CRISPRCasIC,
  title={CRISPR-Cas IIIA Csm 6 CA RF Domain Is a Ring Nuclease Triggering Stepwise cA 4 Cleavage with ApA > p Formation Terminating RNase Activity Graphical},
  author={N. Jia and Roger A. Jones and G. Yang and O. Ouerfelli and D. Patel},
  year={2019}
}

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References

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TLDR
It is demonstrated that the Csm6 CARF domain is a ring nuclease, whereby bound cA4 is stepwise cleaved initially to ApApApA >p and subsequently to ApA>p in its CARFdomain-binding pocket, with such cleavage bursts using a timer mechanism to regulate the RNase activity of the CSm6 HEPN domain. Expand
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