CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

  title={CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering},
  author={Daniel J. Dickinson and Bob Goldstein},
The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome… CONTINUE READING
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