CRISPR–Cas12b enables efficient plant genome engineering

  title={CRISPR–Cas12b enables efficient plant genome engineering},
  author={Meiling Ming and Qiurong Ren and Changtian Pan and Yao He and Yingxiao Zhang and Shishi Liu and Zhaohui Zhong and Jiaheng Wang and Aimee A. Malzahn and Jun Wu and Xuelian Zheng and Yong Zhang and Yiping Qi},
  journal={Nature Plants},
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also… 

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