CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.
@article{Lee2015CRISPRCas9mediatedGE,
title={CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.},
author={Jae Seong Lee and Lise Marie Grav and Nathan E. Lewis and Helene Faustrup Kildegaard},
journal={Biotechnology journal},
year={2015},
volume={10 7},
pages={
979-94
}
}- Published 2015 in Biotechnology journal
DOI:10.1002/biot.201500082
Chinese hamster ovary (CHO) cells are the most widely used production host for therapeutic proteins. With the recent emergence of CHO genome sequences, CHO cell line engineering has taken on a new aspect through targeted genome editing. The bacterial clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system enables rapid, easy and efficient engineering of mammalian genomes. It has a wide range of applications from modification of individual… CONTINUE READING
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