CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.

@article{Lee2015CRISPRCas9mediatedGE,
  title={CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.},
  author={Jae Seong Lee and Lise Marie Grav and Nathan E. Lewis and Helene Faustrup Kildegaard},
  journal={Biotechnology journal},
  year={2015},
  volume={10 7},
  pages={
          979-94
        }
}
Chinese hamster ovary (CHO) cells are the most widely used production host for therapeutic proteins. With the recent emergence of CHO genome sequences, CHO cell line engineering has taken on a new aspect through targeted genome editing. The bacterial clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system enables rapid, easy and efficient engineering of mammalian genomes. It has a wide range of applications from modification of individual… CONTINUE READING

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