CRISPR/Cas9 library screening for drug target discovery

  title={CRISPR/Cas9 library screening for drug target discovery},
  author={Morito Kurata and Kouhei Yamamoto and Branden S. Moriarity and Masanobu Kitagawa and David A. Largaespada},
  journal={Journal of Human Genetics},
CRISPR/Cas9-based tools have rapidly developed in recent years. These include CRISPR-based gene activation (CRISPRa) or inhibition (CRISPRi), for which there are libraries. CRISPR libraries for loss of function have been widely used to identify new biological mechanisms, such as drug resistance and cell survival signals. CRISPRa is highly useful in screening for gain of functions, and CRISPRi is a more powerful tool than RNA interference (RNAi) libraries in screening for loss of functions… 

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