CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

  title={CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii},
  author={Sung-Eun Shin and Jong-Min Lim and Hyun Gi Koh and Eun Kyung Kim and Nam Kyu Kang and Seungjib Jeon and Sohee Kwon and Won-Sub Shin and Bongsoo Lee and Kwon Hwangbo and Jeong Eun Kim and Sung Hyeok Ye and Jae-Young Yun and Hogyun Seo and Hee-Mock Oh and Kyung-Jin Kim and Jin-Soo Kim and Won-joong Jeong and Yong Keun Chang and Byeong-Ryool Jeong},
  booktitle={Scientific reports},
Genome editing is crucial for genetic engineering of organisms for improved traits, particularly in microalgae due to the urgent necessity for the next generation biofuel production. The most advanced CRISPR/Cas9 system is simple, efficient and accurate in some organisms; however, it has proven extremely difficult in microalgae including the model alga Chlamydomonas. We solved this problem by delivering Cas9 ribonucleoproteins (RNPs) comprising the Cas9 protein and sgRNAs to avoid cytotoxicity… CONTINUE READING
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