CRISPR/Cas9 in Genome Editing and Beyond.

@article{Wang2016CRISPRCas9IG,
  title={CRISPR/Cas9 in Genome Editing and Beyond.},
  author={Haifeng Wang and Marie La Russa and Lei S. Qi},
  journal={Annual review of biochemistry},
  year={2016},
  volume={85},
  pages={
          227-64
        }
}
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has made sequence-specific gene editing several magnitudes easier. The nuclease-deactivated form of Cas9… 

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